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rapr p38 expression  (Addgene inc)


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    Addgene inc rapr p38 expression
    <t>p38</t> MAPK mediates basal suppression of the number of inhibitory synapses. A Representative processed confocal images and B bar graph showing the p38 antagonist SB203,580 (10 μM) alone increases the number of intrabody-labeled gephyrin puncta (24 h) relative to untreated (Unpaired two-tailed t-test; t(16)=2.21, p=0.04, n=9; data from 3 independent cell preparations). C SB202474 (10 μM), an inactive analog of SB203580, does not affect the gp120-induced (600 pM) increase in the number of intrabody-labeled gephyrin puncta (24 h)(Unpaired two-tailed t-test; t(18)=0.004, p= 1, n=10; data from 3 independent cell preparations). D The number of intrabody-labeled gephyrin puncta is increased by 24 h treatment with SB239063 (10 μM), a p38 MAPK inhibitor (Unpaired two-tailed t-test; t(14)=4.95, p=0.0002, n=8; data from 3 independent cell preparations). E Treatment with rapamycin (200 nM) decreases the number of inhibitory synapses in cells expressing RapRp38 (Unpaired two-tailed t-test, t(14)=3.71, p=0.002; data from 3 independent cell preparations) but not untreated cells (Unpaired two-tailed t-test, t(14)=0.43, p=0.7; data from 3 independent cell preparations) (n=8). Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<.001 compared to control (n=number of individual dishes in each group).
    Rapr P38 Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapr+p38+expression/pCMV5-Flag-RapR-p38+(Plasmid+%2325935)/pmc06379117-64-3-15
    Average 91 stars, based on 2 article reviews
    rapr p38 expression - by Bioz Stars, 2026-11
    91/100 stars

    Images

    1) Product Images from "HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK"

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK

    Journal: Journal of neurochemistry

    doi: 10.1111/jnc.14640

    p38 MAPK mediates basal suppression of the number of inhibitory synapses. A Representative processed confocal images and B bar graph showing the p38 antagonist SB203,580 (10 μM) alone increases the number of intrabody-labeled gephyrin puncta (24 h) relative to untreated (Unpaired two-tailed t-test; t(16)=2.21, p=0.04, n=9; data from 3 independent cell preparations). C SB202474 (10 μM), an inactive analog of SB203580, does not affect the gp120-induced (600 pM) increase in the number of intrabody-labeled gephyrin puncta (24 h)(Unpaired two-tailed t-test; t(18)=0.004, p= 1, n=10; data from 3 independent cell preparations). D The number of intrabody-labeled gephyrin puncta is increased by 24 h treatment with SB239063 (10 μM), a p38 MAPK inhibitor (Unpaired two-tailed t-test; t(14)=4.95, p=0.0002, n=8; data from 3 independent cell preparations). E Treatment with rapamycin (200 nM) decreases the number of inhibitory synapses in cells expressing RapRp38 (Unpaired two-tailed t-test, t(14)=3.71, p=0.002; data from 3 independent cell preparations) but not untreated cells (Unpaired two-tailed t-test, t(14)=0.43, p=0.7; data from 3 independent cell preparations) (n=8). Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<.001 compared to control (n=number of individual dishes in each group).
    Figure Legend Snippet: p38 MAPK mediates basal suppression of the number of inhibitory synapses. A Representative processed confocal images and B bar graph showing the p38 antagonist SB203,580 (10 μM) alone increases the number of intrabody-labeled gephyrin puncta (24 h) relative to untreated (Unpaired two-tailed t-test; t(16)=2.21, p=0.04, n=9; data from 3 independent cell preparations). C SB202474 (10 μM), an inactive analog of SB203580, does not affect the gp120-induced (600 pM) increase in the number of intrabody-labeled gephyrin puncta (24 h)(Unpaired two-tailed t-test; t(18)=0.004, p= 1, n=10; data from 3 independent cell preparations). D The number of intrabody-labeled gephyrin puncta is increased by 24 h treatment with SB239063 (10 μM), a p38 MAPK inhibitor (Unpaired two-tailed t-test; t(14)=4.95, p=0.0002, n=8; data from 3 independent cell preparations). E Treatment with rapamycin (200 nM) decreases the number of inhibitory synapses in cells expressing RapRp38 (Unpaired two-tailed t-test, t(14)=3.71, p=0.002; data from 3 independent cell preparations) but not untreated cells (Unpaired two-tailed t-test, t(14)=0.43, p=0.7; data from 3 independent cell preparations) (n=8). Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<.001 compared to control (n=number of individual dishes in each group).

    Techniques Used: Labeling, Two Tailed Test, Expressing, Control

    Related Articles

    Plasmid Preparation:

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK.
    Article Snippet: The expression vector for synapsin driven tdTomato was generated by inserting tdTomato from pLVX-tdTomato-N1 (catalog number: 632563; Clontech-Takara Bio, Mountain View, CA, USA) into the pSyn backbone of pSyn-PSD95GFP provided by Kirill Martemyanov (Scripps Research Institute, Jupiter, FL, USA). .. The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935). ..

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK
    Article Snippet: The expression vector for synapsin driven tdTomato was generated by inserting tdTomato from pLVX-tdTomato-N1 (catalog number: 632563; Clontech-Takara Bio, Mountain View, CA, USA) into the pSyn backbone of pSyn-PSD95-GFP provided by Kirill Martemyanov (Scripps Research Institute, Jupiter, FL, USA). .. The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935). ..

    Expressing:

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK.
    Article Snippet: The expression vector for synapsin driven tdTomato was generated by inserting tdTomato from pLVX-tdTomato-N1 (catalog number: 632563; Clontech-Takara Bio, Mountain View, CA, USA) into the pSyn backbone of pSyn-PSD95GFP provided by Kirill Martemyanov (Scripps Research Institute, Jupiter, FL, USA). .. The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935). ..

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK
    Article Snippet: The expression vector for synapsin driven tdTomato was generated by inserting tdTomato from pLVX-tdTomato-N1 (catalog number: 632563; Clontech-Takara Bio, Mountain View, CA, USA) into the pSyn backbone of pSyn-PSD95-GFP provided by Kirill Martemyanov (Scripps Research Institute, Jupiter, FL, USA). .. The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935). ..

    Generated:

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK.
    Article Snippet: The expression vector for synapsin driven tdTomato was generated by inserting tdTomato from pLVX-tdTomato-N1 (catalog number: 632563; Clontech-Takara Bio, Mountain View, CA, USA) into the pSyn backbone of pSyn-PSD95GFP provided by Kirill Martemyanov (Scripps Research Institute, Jupiter, FL, USA). .. The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935). ..

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK
    Article Snippet: The expression vector for synapsin driven tdTomato was generated by inserting tdTomato from pLVX-tdTomato-N1 (catalog number: 632563; Clontech-Takara Bio, Mountain View, CA, USA) into the pSyn backbone of pSyn-PSD95-GFP provided by Kirill Martemyanov (Scripps Research Institute, Jupiter, FL, USA). .. The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935). ..



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    rapr p38 expression  (Addgene inc)
    91
    Addgene inc rapr p38 expression
    <t>p38</t> MAPK mediates basal suppression of the number of inhibitory synapses. A Representative processed confocal images and B bar graph showing the p38 antagonist SB203,580 (10 μM) alone increases the number of intrabody-labeled gephyrin puncta (24 h) relative to untreated (Unpaired two-tailed t-test; t(16)=2.21, p=0.04, n=9; data from 3 independent cell preparations). C SB202474 (10 μM), an inactive analog of SB203580, does not affect the gp120-induced (600 pM) increase in the number of intrabody-labeled gephyrin puncta (24 h)(Unpaired two-tailed t-test; t(18)=0.004, p= 1, n=10; data from 3 independent cell preparations). D The number of intrabody-labeled gephyrin puncta is increased by 24 h treatment with SB239063 (10 μM), a p38 MAPK inhibitor (Unpaired two-tailed t-test; t(14)=4.95, p=0.0002, n=8; data from 3 independent cell preparations). E Treatment with rapamycin (200 nM) decreases the number of inhibitory synapses in cells expressing RapRp38 (Unpaired two-tailed t-test, t(14)=3.71, p=0.002; data from 3 independent cell preparations) but not untreated cells (Unpaired two-tailed t-test, t(14)=0.43, p=0.7; data from 3 independent cell preparations) (n=8). Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<.001 compared to control (n=number of individual dishes in each group).
    Rapr P38 Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapr+p38+expression/pCMV5-Flag-RapR-p38+(Plasmid+%2325935)/pmc06379117-64-3-15
    Average 91 stars, based on 1 article reviews
    rapr p38 expression - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    p38 MAPK mediates basal suppression of the number of inhibitory synapses. A Representative processed confocal images and B bar graph showing the p38 antagonist SB203,580 (10 μM) alone increases the number of intrabody-labeled gephyrin puncta (24 h) relative to untreated (Unpaired two-tailed t-test; t(16)=2.21, p=0.04, n=9; data from 3 independent cell preparations). C SB202474 (10 μM), an inactive analog of SB203580, does not affect the gp120-induced (600 pM) increase in the number of intrabody-labeled gephyrin puncta (24 h)(Unpaired two-tailed t-test; t(18)=0.004, p= 1, n=10; data from 3 independent cell preparations). D The number of intrabody-labeled gephyrin puncta is increased by 24 h treatment with SB239063 (10 μM), a p38 MAPK inhibitor (Unpaired two-tailed t-test; t(14)=4.95, p=0.0002, n=8; data from 3 independent cell preparations). E Treatment with rapamycin (200 nM) decreases the number of inhibitory synapses in cells expressing RapRp38 (Unpaired two-tailed t-test, t(14)=3.71, p=0.002; data from 3 independent cell preparations) but not untreated cells (Unpaired two-tailed t-test, t(14)=0.43, p=0.7; data from 3 independent cell preparations) (n=8). Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<.001 compared to control (n=number of individual dishes in each group).

    Journal: Journal of neurochemistry

    Article Title: HIV gp120-induced neuroinflammation potentiates NMDA receptors to overcome basal suppression of inhibitory synapses by p38 MAPK

    doi: 10.1111/jnc.14640

    Figure Lengend Snippet: p38 MAPK mediates basal suppression of the number of inhibitory synapses. A Representative processed confocal images and B bar graph showing the p38 antagonist SB203,580 (10 μM) alone increases the number of intrabody-labeled gephyrin puncta (24 h) relative to untreated (Unpaired two-tailed t-test; t(16)=2.21, p=0.04, n=9; data from 3 independent cell preparations). C SB202474 (10 μM), an inactive analog of SB203580, does not affect the gp120-induced (600 pM) increase in the number of intrabody-labeled gephyrin puncta (24 h)(Unpaired two-tailed t-test; t(18)=0.004, p= 1, n=10; data from 3 independent cell preparations). D The number of intrabody-labeled gephyrin puncta is increased by 24 h treatment with SB239063 (10 μM), a p38 MAPK inhibitor (Unpaired two-tailed t-test; t(14)=4.95, p=0.0002, n=8; data from 3 independent cell preparations). E Treatment with rapamycin (200 nM) decreases the number of inhibitory synapses in cells expressing RapRp38 (Unpaired two-tailed t-test, t(14)=3.71, p=0.002; data from 3 independent cell preparations) but not untreated cells (Unpaired two-tailed t-test, t(14)=0.43, p=0.7; data from 3 independent cell preparations) (n=8). Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<.001 compared to control (n=number of individual dishes in each group).

    Article Snippet: The plasmid for RapR-p38 expression (pCMV5-Flag-RapR-p38) was generated in Klaus Hahn’s laboratory and obtained from Addgene (catalog number: 25935).

    Techniques: Labeling, Two Tailed Test, Expressing, Control